DNA

Part:BBa_J435330:Design

Designed by: Open Bioeconomy   Group: FreeGenes   (2023-05-09)


high copy (pUC) ori/KanR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 56
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 56
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1758
    Illegal SapI.rc site found at 6


Design Notes

Unknown


Source

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

Author: Open Bioeconomy Lab

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003376.html

References